Portable fluorescence polarization analyzer for on-site multisample immunoassay

Ayano Nakamura, Osamu Wakao, Ken Satou, Mitsutoshi Aoyagi, Kazuhiko Nishimura, Chikaaki Mizokuchi, Ken Sumiyoshi, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Koji Shigemura, Akihide Hibara, Manabu Tokeshi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports on a portable fluorescence polarization (FP) analyzer capable of on-site multisample immunoassay. The FP analyzer is small-size (W × D × H = 65 cm3), low cost (< $5,000), and high throughput (96 samples simultaneously). Using this analyzer, we demonstrated FP immunoassay (FPIA) for deoxynivalenol (DON), one of several mycotoxins that frequently infect wheat and other grains, spiked into wheat samples. The assay with the compact FP analyzer had sufficient accuracy to quantify DON in wheat in comparison with LC-MS/MS. Furthermore, multisample immunoassay was conducted by using a microdevice with 96 chambers.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1393-1394
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: 2019 Oct 272019 Oct 31

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period19/10/2719/10/31

Keywords

  • Deoxynivalenol
  • Fluorescence Polarization
  • Immunoassay
  • Mycotoxin

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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