Porphyromonas pogonae sp. nov., an anaerobic but low concentration oxygen adapted coccobacillus isolated from lizards (Pogona vitticeps) or human clinical specimens, and emended description of the genus Porphyromonas Shah and Collins 1988

Yoshiaki Kawamura, Saki Kuwabara, Stephen A. Kania, Hisayuki Kato, Manami Hamagishi, Nagatoshi Fujiwara, Takuichi Sato, Junko Tomida, Kaori Tanaka, David A. Bemis

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

During the process of identifying a Gram-negative coccobacillus isolated from a human clinical specimen, we found that the isolate's 16S rRNA gene had very close sequence identity with that of a variant Porphyromonas isolated from polymicrobial infections in the central bearded dragon, a species of lizard [2]. The 16S rRNA gene sequences of the human isolate and of six isolates from lizards were nearly identical (99.9-100%). Phylogenetic analysis placed all of these isolates in a single phylogenetic cluster well separated from other species in the genus Porphyromonas. The closest species was Porphyromonas catoniae with 90.7-90.9% sequence identity, although there was less than 6% DNA similarity between the P. catoniae type strain and our representative isolates from lizards (PAGU 1787T) and human (PAGU 1776). These isolates could grow under anaerobic or microaerobic conditions (6% O2 atmosphere). The isolates were positive for catalase and very strong β-hemolytic activity, but did not show black or brown pigmentation. Biochemically, the isolates could be differentiated from closely related species by pyroglutamic acid arylamidase and glycine arylamidase activity, and some others. The fermentation products mainly included succinic acid and propionic acid. The major fatty acids detected in cells of the isolates were iso-C15:0, anteiso-C15:0, and 3OH-iso-C17:0. The G+C content was 43.0±0.62mol%. The species name Porphyromonas pogonae sp. nov. is proposed for these isolates with the type strain of PAGU 1787T (=MI 10-1288T=JCM 19732T=ATCC BAA-2643T).

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalSystematic and Applied Microbiology
Volume38
Issue number2
DOIs
Publication statusPublished - 2015 Mar 1

Keywords

  • 16S rRNA sequence
  • Central bearded dragons
  • DNA-DNA hybridization
  • Human clinical specimen
  • Pogona vitticeps
  • Porphyromonas

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

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