Porphyromonas gingivalis lipopolysaccharides induce maturation of dendritic cells with CD14+CD16+ phenotype

Sousuke Kanaya, Eiji Nemoto, Ogawa Tomohiko, Hidetoshi Shimauchi

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Primary immune responses are initiated by dendritic cells (DC) that inform naive T helper cells about invading pathogens. DC undergo sequential events leading to irreversible maturation upon bacterial stimulation. To investigate the responses of DC during periodontal infection, we studied the effects of LPS from Porphyromonas gingivalis on DC. DC generated from human peripheral monocytes by culture with IL-4 and GM-CSF were incubated with P. gingivalis LPS (Pg LPS) or Escherichia coli LPS (Ec LPS). Flow cytometry and real-time quantitative RT-PCR analysis revealed that Pg LPS, but not Ec LPS, preferentially upregulated CD14 and CD16 expression at protein and mRNA levels. Furthermore, Pg LPS preferentially induced the secretion of soluble CD14. CD1a, HLA-DR and CD54 were highly expressed on DC stimulated with both kinds of LPS; however, CD40, CD80, CD83 and CD86 expression on Pg LPS-stimulated DC was lower than on Ec LPS-stimulated DC. With regard to IL-6, IL-8, IL-10, IL-12 and RANTES production from DC and allogeneic T cell proliferation, Pg LPS was a weaker stimulator than Ec LPS. These results suggested that Pg LPS triggers maturation of DC with unique characteristics, which exhibited weak immunostimulatory activity and may contribute to induction of chronic inflammation at the site of periodontal infection.

Original languageEnglish
Pages (from-to)1451-1460
Number of pages10
JournalEuropean Journal of Immunology
Volume34
Issue number5
DOIs
Publication statusPublished - 2004 May 1

Keywords

  • Cellular differentiation
  • Cytokine
  • Human dentritic cells
  • Lipopolysaccharide
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Immunology

Fingerprint

Dive into the research topics of 'Porphyromonas gingivalis lipopolysaccharides induce maturation of dendritic cells with CD14+CD16+ phenotype'. Together they form a unique fingerprint.

Cite this