Porcine brain natriuretic peptide-like immunoreactivity in rat tissues

Masahiko Sone, Kazuhito Totsune, Kazuhiro Takahashi, Makoto Ohneda, Keiichi Itoi, Osamu Murakami, Kaoru Yoshinaga, Toraichi Mouri

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The presence of immunoreactive porcine brain natriuretic peptide in rat tissues was studied with a specific radioimmunoassay for porcine brain natriuretic peptide-26. The cross-reactivity of the antiserum used was less than 0.001% with rat atrial natriuretic peptide, rat brain natriuretic peptide-32 and rat brain natriuretic peptide-45. Immunoreactive porcine brain natriuretic peptide was detectable in various tissues of the rat, and high concentrations of immunoreactive porcine brain natriuretic peptide were found in the brain and cardiac atrium, with the highest level in the hypothalamus (159±30 fmol/gram wet tissue, mean±SEM, n=4). Reverse phase high performance liquid chromatography showed that the immunoreactive porcine brain natriuretic peptide of the whole brain and heart extracts eluted mainly at an identical position to synthetic porcine brain natriuretic peptide-26. These findings indicate that porcine brain natriuretic peptide-like substance, distinct from rat brain natriuretic peptide, is present in high concentrations in the rat brain and cardiac atrium.

Original languageEnglish
Pages (from-to)1333-1335
Number of pages3
Issue number6
Publication statusPublished - 1991


  • Atrial natriuretic peptide
  • Brain natriuretic peptide
  • Chromatography
  • Radioimmunoassay
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience


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