TY - JOUR
T1 - Porcine brain natriuretic peptide-like immunoreactivity in rat tissues
AU - Sone, Masahiko
AU - Totsune, Kazuhito
AU - Takahashi, Kazuhiro
AU - Ohneda, Makoto
AU - Itoi, Keiichi
AU - Murakami, Osamu
AU - Yoshinaga, Kaoru
AU - Mouri, Toraichi
PY - 1991
Y1 - 1991
N2 - The presence of immunoreactive porcine brain natriuretic peptide in rat tissues was studied with a specific radioimmunoassay for porcine brain natriuretic peptide-26. The cross-reactivity of the antiserum used was less than 0.001% with rat atrial natriuretic peptide, rat brain natriuretic peptide-32 and rat brain natriuretic peptide-45. Immunoreactive porcine brain natriuretic peptide was detectable in various tissues of the rat, and high concentrations of immunoreactive porcine brain natriuretic peptide were found in the brain and cardiac atrium, with the highest level in the hypothalamus (159±30 fmol/gram wet tissue, mean±SEM, n=4). Reverse phase high performance liquid chromatography showed that the immunoreactive porcine brain natriuretic peptide of the whole brain and heart extracts eluted mainly at an identical position to synthetic porcine brain natriuretic peptide-26. These findings indicate that porcine brain natriuretic peptide-like substance, distinct from rat brain natriuretic peptide, is present in high concentrations in the rat brain and cardiac atrium.
AB - The presence of immunoreactive porcine brain natriuretic peptide in rat tissues was studied with a specific radioimmunoassay for porcine brain natriuretic peptide-26. The cross-reactivity of the antiserum used was less than 0.001% with rat atrial natriuretic peptide, rat brain natriuretic peptide-32 and rat brain natriuretic peptide-45. Immunoreactive porcine brain natriuretic peptide was detectable in various tissues of the rat, and high concentrations of immunoreactive porcine brain natriuretic peptide were found in the brain and cardiac atrium, with the highest level in the hypothalamus (159±30 fmol/gram wet tissue, mean±SEM, n=4). Reverse phase high performance liquid chromatography showed that the immunoreactive porcine brain natriuretic peptide of the whole brain and heart extracts eluted mainly at an identical position to synthetic porcine brain natriuretic peptide-26. These findings indicate that porcine brain natriuretic peptide-like substance, distinct from rat brain natriuretic peptide, is present in high concentrations in the rat brain and cardiac atrium.
KW - Atrial natriuretic peptide
KW - Brain natriuretic peptide
KW - Chromatography
KW - Radioimmunoassay
KW - Rat
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U2 - 10.1016/0196-9781(91)90216-C
DO - 10.1016/0196-9781(91)90216-C
M3 - Article
C2 - 1815220
AN - SCOPUS:0026316502
VL - 12
SP - 1333
EP - 1335
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 6
ER -