Polyubiquitin promoter-based binary vectors for overexpression and gene silencing in Lotus japonicus

Takaki Maekawa, Mitsumasa Kusakabe, Yoshikazu Shimoda, Shusei Sato, Satoshi Tabata, Yoshikatsu Murooka, Makoto Hayashi

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.

Original languageEnglish
Pages (from-to)375-382
Number of pages8
JournalMolecular Plant-Microbe Interactions
Volume21
Issue number4
DOIs
Publication statusPublished - 2008 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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