Polysaccharide-hair cationic polypeptide nanogels: Self-assembly and enzymatic polymerization of amylose primer-modified cholesteryl poly(L-lysine)

Nobuyuki Morimoto, Mio Yamazaki, Junko Tamada, Kazunari Akiyoshi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

In this study, we prepared a new associating polymer, ChMaPLL, by the substitution of the poly(l-lysine) moiety with oligosaccharide amylose primer and cholesterol. ChMaPLL formed positively charged polypeptide nanogels (∼50 nm) via self-assembly in water. The nanogels showed a secondary structural transition to an α-helix structure induced by poly(l-lysine) in response to an increase in pH. Oligosaccharides of the nanogels reacted with the phosphorylase a enzyme. Amylose-conjugated nanogels were obtained by enzymatic polymerization. The elongation of the saccharide chain shielded the positive charge of the nanogels. The multiresponsive polysaccharide-polypeptide hybrid nanogels might prove to be useful in the areas of biotechnology and biomedicine.

Original languageEnglish
Pages (from-to)7509-7514
Number of pages6
JournalLangmuir
Volume29
Issue number24
DOIs
Publication statusPublished - 2013 Jun 18

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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