Abstract
In order to study the polyphosphate (PolyP) metabolism in the acidophilic alga, the effect of rapid change of external phosphate concentration (so-called Pi stress condition) on the distribution of PolyP in Chlamydomonas acidophila KT-1 was investigated by fluorescent microscopy, in vivo 31P NMR spectroscopy, and enzymatic quantification. The PolyP granules inside of C. acidophila KT-1 cells were observed by microscopy with a fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI). Cells transferred from phosphate (Pi) deficient to Pi rich conditions were contained a large amount of PolyP and an additional new PolyP stain was observed around the cell surface. In comparison to a neutrophilic alga Chlamydomonas reinhardtii C-9, the total amount of PolyP in C. acidophila KT-1 was 2.5 times smaller. The lower content of PolyP in C. acidophila KT-1 was supposed to be attributable to the specific properties of its cell membrane, which limited to incorporate phosphate from the environment. Nevertheless, the trend of degradation and re-synthesis of PolyP in C. acidophila KT-1 was similar to that of C. reinhardtii C-9 under Pi stress conditions with absolute PolyP amount being affected by the total phosphate in the cells.
Original language | English |
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Pages (from-to) | 307-313 |
Number of pages | 7 |
Journal | Plant Science |
Volume | 170 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2006 Feb 1 |
Keywords
- Acidophilic algae
- Chlamydomonas
- Fluorescent microscopy
- In vivo P NMR
- Phosphate deficient condition
- Polyphosphate metabolism
ASJC Scopus subject areas
- Genetics
- Agronomy and Crop Science
- Plant Science