Polyphosphate dynamics in mycorrhizal roots during colonization of an arbuscular mycorrhizal fungus

Ryo Ohtomo, Masanori Saito

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

• Inorganic polyphosphate (poly P) has been considered to be a translocatable form of phosphate (Pi) in arbuscular mycorrhizal fungi (AMF). Here we examined time-course changes in poly P content during the AMF colonization process. • Onion (Allium cepa) plants were cultured with or without inoculation with Gigaspora margarita for 2-8 wk with periodic sampling. Poly P in the extracts, purified through gel filtration, was quantified by the reverse reaction of polyphosphate kinase. • The length of poly P in mycorrhizal roots appeared to be shorter than in extraradical hyphae or in spores of the AMF, indicating that AMF depolymerize poly P before providing Pi to the host. The poly P content increased as colonization proceeded, and was highly correlated with the weight of the colonized roots. • These results support the model that AMF supply Pi to the host through the poly P pool, and that the poly P content of a mycorrhizal root can be a good indicator of the Pi-supplying activity of AMF.

Original languageEnglish
Pages (from-to)571-578
Number of pages8
JournalNew Phytologist
Volume167
Issue number2
DOIs
Publication statusPublished - 2005 Aug

Keywords

  • Arbuscular mycorrhizal fungi (AMF)
  • Inorganic phosphate (P), polyphosphate (poly P)
  • Polyphosphate kinase (PPK)

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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