Polyphasic identification of a thermophilic bacterium from geyser of Cisolok, Indonesia

Abinubli Tariswafi Mawarid, Wellyzar Sjamsuridzal, Shuhei Yabe, Iman Santoso, Fitria Ningsih, Dafina Ghossani Nurlaili, Ariyanti Oetari, Akira Yokota

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

A thermophilic bacterium strain UICC B-76 was isolated from litter in geyser of Cisolok, West Java, Indonesia by using The International Streptomyces Project (ISP1) medium. The strain UICC B-76 was able to hydrolyze various substrates such as casein, cellulose, gelatin, starch, and xylan, at 50 °C, and decolorize Remazol Brilliant Blue R (RBBR) at 50 °C, an indicator for ligninolytic degradation ability. These abilities of strain UICC B-76 suggest its potential for industrial applications. The aim of this study was to identify strain UICC B-76 based on phenotypic and genotypic characterizations. Strain UICC B-76 was Gram-positive, spore-forming, aerobic and facultative anaerobic, and rod-shaped with flagella. The strain grew at 30 °C to 60 °C (optimum 50 °C) and pH 7.0 but can not withstand temperature of 70 °C as well as pH 4.0 and 9.0. Strain UICC B-76 could not grow in more than 1% NaCl. This strain has menaquinone 7 (MK-7) as the major quinone system; cell wall fatty acids consisted of Iso-C15:0 (68.56%), Iso-C17:0 (12.48%), and Anteiso-C15:0 (8.45%); and the main amino acids of the cell wall was meso-diaminopimelic acid (meso-DAP). Phylogenetic analysis based on the full sequence of 16S rRNA gene (1450 nt) indicated that strain UICC B-76 belongs to the genus Brevibacillus (Family Paenibacillaceae, Order Bacillales, Class Bacilli, Phylum Firmicutes). Strain UICC B-76 was closely related with type strains of Brevibacillus parabrevis and those of B. agri, however, strain UICC B-76 was separated from both species. The 16S rDNA sequence similarity of strain UICC B-76 showed relatively low sequence homology value, less than 99%, to the type of strains B. parabrevis (ranged between 98.13% and 98.20%) and B. agri (98.13%). Moreover, the G+C content of strain UICC B-76 (55 mol%) differs with B. parabrevis (51.8-52.2 mol%) and B. agri (53.5 mol%). This result indicated that strain UICC B-76 may represent a new species of Brevibacillus. Hybridization of DNA among strain UICC B-76, B. parabrevis and B. agri is required to clarify the proposal of a new species.

Original languageEnglish
Title of host publicationInternational Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
Subtitle of host publicationProceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences
EditorsTerry Mart, Djoko Triyono
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413764
DOIs
Publication statusPublished - 2016 Apr 19
Event1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 - Depok, Indonesia
Duration: 2015 Nov 32015 Nov 4

Publication series

NameAIP Conference Proceedings
Volume1729
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
Country/TerritoryIndonesia
CityDepok
Period15/11/315/11/4

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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