TY - JOUR
T1 - Polypeptide synthesis using an expressed peptide as a building block via the thioester method
AU - Kawakami, Toru
AU - Hasegawa, Koki
AU - Teruya, Kenta
AU - Akaji, Kenichi
AU - Horiuchi, Masataka
AU - Inagaki, Fuyuhiko
AU - Kurihara, Yasuyuki
AU - Uesugi, Seiichi
AU - Aimoto, Saburo
N1 - Funding Information:
This research was partly supported by the Grant-in-Aid for Scientific Research on Priority Areas No. 10179103 from the Ministry of Education, Science, Sports and Culture, Japan.
PY - 2000/4/8
Y1 - 2000/4/8
N2 - An expressed peptide was proven to be useful as a building block in the synthesis of a polypeptide via the thioester method. A partially protected peptide segment, [Lys(Boc)15,25,31,48,57,68,80,95]- Max(14-101), was prepared via transamination of an N-terminal amino group from p21Max(13-101), which was obtained by expression using Escherichia coli. This peptide was utilized in the synthesis of [Ser(PO3H2)2,11]- p21Max(1-101) via condensation with the peptide thioester, Boc- [Ser(PO3H2)2,11]-p21Max(1-13)-SCH2CH2CO-Leu-OH. (C) 2000 Elsevier Science Ltd.
AB - An expressed peptide was proven to be useful as a building block in the synthesis of a polypeptide via the thioester method. A partially protected peptide segment, [Lys(Boc)15,25,31,48,57,68,80,95]- Max(14-101), was prepared via transamination of an N-terminal amino group from p21Max(13-101), which was obtained by expression using Escherichia coli. This peptide was utilized in the synthesis of [Ser(PO3H2)2,11]- p21Max(1-101) via condensation with the peptide thioester, Boc- [Ser(PO3H2)2,11]-p21Max(1-13)-SCH2CH2CO-Leu-OH. (C) 2000 Elsevier Science Ltd.
KW - Condensations
KW - Peptide building blocks
KW - Peptides and polypeptides
KW - Thioesters
UR - http://www.scopus.com/inward/record.url?scp=0034620934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034620934&partnerID=8YFLogxK
U2 - 10.1016/S0040-4039(00)00232-X
DO - 10.1016/S0040-4039(00)00232-X
M3 - Article
AN - SCOPUS:0034620934
VL - 41
SP - 2625
EP - 2628
JO - Tetrahedron Letters
JF - Tetrahedron Letters
SN - 0040-4039
IS - 15
ER -