Polymorphisms and functional differences in aryl hydrocarbon receptors (AhR) in Japanese field mice, Apodemus speciosus

Hiroko Ishiniwa, Kazuhiro Sogawa, Ken ichi Yasumoto, Tsuneo Sekijima

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Dioxins, which are unintentionally generated toxic pollutants, exert a variety of adverse effects on organisms. The majority of these effects, which include teratogenesis, immunosuppression, tumor promotion, and endocrine disruption, are mediated through aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor. Genetic variations in AhR result in different survivability under exposure to dioxin contamination, which might affect the genetic structure of wildlife populations through differential susceptibility to dioxin exposure. The aim of this study was to clarify the polymorphisms of AhR in Japanese field mice, Apodemus speciosus, and their functional differences in order to develop a molecular indicator for dioxin sensitivity.Wild Japanese field mice had abundant polymorphisms in AhR coding region. Seventy-one single nucleotide polymorphisms, 27 of which occur amino acid substitutions, and consequently 49 alleles were identified in 63 individuals. In the functional analysis of AhR variants using transient reporter assays, a Gln to Arg mutation at amino acid 799 exhibited a significant decrease in the level of transactivational properties (p=0.015) which might modify the dioxin susceptibility of an individual.

    Original languageEnglish
    Pages (from-to)280-289
    Number of pages10
    JournalEnvironmental Toxicology and Pharmacology
    Volume29
    Issue number3
    DOIs
    Publication statusPublished - 2010 May

    Keywords

    • Apodemus speciosus
    • Aryl hydrocarbon receptor
    • DNA polymorphism
    • Dioxin
    • Gene-environment interaction
    • Genetic variation
    • Japanese field mouse

    ASJC Scopus subject areas

    • Toxicology
    • Pharmacology
    • Health, Toxicology and Mutagenesis

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