TY - JOUR
T1 - Polymorphic minisatellites in the mitochondrial DNAs of Oryza and Brassica
AU - Honma, Yujiro
AU - Yoshida, Yu
AU - Terachi, Toru
AU - Toriyama, Kinya
AU - Mikami, Tetsuo
AU - Kubo, Tomohiko
N1 - Funding Information:
Acknowledgments The authors wish to thank Prof. Dr. Takeshi Nishio and the National Institute of Genetics for providing plant materials, and Prof. Dr. Hiroshi Yamagishi for valuable comments. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology, Japan; the Private University Strategic Research Foundation Support Program; and the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN).
Funding Information:
Plant materials used in this study are listed in Tables 1 and 2. The wild rice accessions were distributed from the National Institute of Genetics supported by the National Bioresource Project, MEXT, Japan (http://www. shigen.nig.ac.jp/rice/oryzabase/wild/coreCollection.jsp). The Brassica accessions used in this study were gifts from Professor Dr. Takeshi Nishio, Tohoku University, Japan.
PY - 2011/8
Y1 - 2011/8
N2 - Polymorphic analyses of angiosperm mitochondrial DNA are rare in comparison with chloroplast DNA, because few target sequences in angiosperm mitochondrial DNA are known. Minisatellites, a tandem array of repeated sequences with a repeat unit of 10 to ~100 bp, are popular target sequences of animal mitochondria, but Beta vulgaris is the only known angiosperm species for which such an analysis has been conducted. From this lack of information, it was uncertain as to whether polymorphic minisatellites existed in other angiosperm species. Ten plant mitochondrial DNAs were found to contain minisatellite-like repeated sequences, most of which were located in intergenic regions but a few occurred in gene coding and intronic regions. Oryza and Brassica accessions were selected as models for the investigation of minisatellite polymorphism because substantial systematic information existed. PCR analysis of 42 Oryza accessions revealed length polymorphisms in four of the five minisatellites. The mitochondrial haplotypes of the 16 Oryza accessions with chromosomal complement (genome) types of CC, BBCC and CCDD were identical but were clearly distinguished from BB-genome accessions, a result consistent with the notion that the cytoplasmic donor parent of the amphidiploid species might be the CC-genome species. Twenty-nine accessions of six major cultivated species of Brassica were classified into five mitochondrial haplotypes based on two polymorphic minisatellites out of six loci. The haplotypes of Brassica juncea and Brassica carinata accessions were identical to Brassica rapa and Brassica nigra accessions, respectively. The haplotypes of Brassica napus accessions were heterogeneous and unique, results that were consistent with previous studies.
AB - Polymorphic analyses of angiosperm mitochondrial DNA are rare in comparison with chloroplast DNA, because few target sequences in angiosperm mitochondrial DNA are known. Minisatellites, a tandem array of repeated sequences with a repeat unit of 10 to ~100 bp, are popular target sequences of animal mitochondria, but Beta vulgaris is the only known angiosperm species for which such an analysis has been conducted. From this lack of information, it was uncertain as to whether polymorphic minisatellites existed in other angiosperm species. Ten plant mitochondrial DNAs were found to contain minisatellite-like repeated sequences, most of which were located in intergenic regions but a few occurred in gene coding and intronic regions. Oryza and Brassica accessions were selected as models for the investigation of minisatellite polymorphism because substantial systematic information existed. PCR analysis of 42 Oryza accessions revealed length polymorphisms in four of the five minisatellites. The mitochondrial haplotypes of the 16 Oryza accessions with chromosomal complement (genome) types of CC, BBCC and CCDD were identical but were clearly distinguished from BB-genome accessions, a result consistent with the notion that the cytoplasmic donor parent of the amphidiploid species might be the CC-genome species. Twenty-nine accessions of six major cultivated species of Brassica were classified into five mitochondrial haplotypes based on two polymorphic minisatellites out of six loci. The haplotypes of Brassica juncea and Brassica carinata accessions were identical to Brassica rapa and Brassica nigra accessions, respectively. The haplotypes of Brassica napus accessions were heterogeneous and unique, results that were consistent with previous studies.
KW - Mitochondrial evolution
KW - Mitochondrial genome
KW - Plant mitochondria
KW - Rapeseed
KW - Rice
KW - Variable number of tandem repeat loci
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U2 - 10.1007/s00294-011-0345-3
DO - 10.1007/s00294-011-0345-3
M3 - Article
C2 - 21562713
AN - SCOPUS:79960919691
VL - 57
SP - 261
EP - 270
JO - Current Genetics
JF - Current Genetics
SN - 0172-8083
IS - 4
ER -