Polymorphic minisatellites in the mitochondrial DNAs of Oryza and Brassica

Yujiro Honma, Yu Yoshida, Toru Terachi, Kinya Toriyama, Tetsuo Mikami, Tomohiko Kubo

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Polymorphic analyses of angiosperm mitochondrial DNA are rare in comparison with chloroplast DNA, because few target sequences in angiosperm mitochondrial DNA are known. Minisatellites, a tandem array of repeated sequences with a repeat unit of 10 to ~100 bp, are popular target sequences of animal mitochondria, but Beta vulgaris is the only known angiosperm species for which such an analysis has been conducted. From this lack of information, it was uncertain as to whether polymorphic minisatellites existed in other angiosperm species. Ten plant mitochondrial DNAs were found to contain minisatellite-like repeated sequences, most of which were located in intergenic regions but a few occurred in gene coding and intronic regions. Oryza and Brassica accessions were selected as models for the investigation of minisatellite polymorphism because substantial systematic information existed. PCR analysis of 42 Oryza accessions revealed length polymorphisms in four of the five minisatellites. The mitochondrial haplotypes of the 16 Oryza accessions with chromosomal complement (genome) types of CC, BBCC and CCDD were identical but were clearly distinguished from BB-genome accessions, a result consistent with the notion that the cytoplasmic donor parent of the amphidiploid species might be the CC-genome species. Twenty-nine accessions of six major cultivated species of Brassica were classified into five mitochondrial haplotypes based on two polymorphic minisatellites out of six loci. The haplotypes of Brassica juncea and Brassica carinata accessions were identical to Brassica rapa and Brassica nigra accessions, respectively. The haplotypes of Brassica napus accessions were heterogeneous and unique, results that were consistent with previous studies.

Original languageEnglish
Pages (from-to)261-270
Number of pages10
JournalCurrent Genetics
Volume57
Issue number4
DOIs
Publication statusPublished - 2011 Aug

Keywords

  • Mitochondrial evolution
  • Mitochondrial genome
  • Plant mitochondria
  • Rapeseed
  • Rice
  • Variable number of tandem repeat loci

ASJC Scopus subject areas

  • Genetics

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