Polymers of 2-methacryloyloxyethyl phosphorylcholine truly work as cell membrane mimic?

Tomoko Mori, Takuya Kubo, Toyohiko J. Konno, Ken Hosoya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have prepared several types of polymers derived from 2-methacryloyloxyethyl phosphorylcholine (MPC) to evaluate whether polymers of MPC work as cell membrane mimic or not. We firstly applied capturing test of target proteins of 4-carboxybenzenesulfonamide (Sul) or ibuprofen (Ibu) as a probe.As the results, the rather hydrophilic polymers based on MPC were able to suppress non-specific binding proteins as expected. Additionally, some of the MPC based polymeric surface was able to capture greater amount of carbonic anhydrase II than those of other polymers, when Sul was utilized as probe. In contrary, all the polymers having PC groups and Ibu probe were unable to capture Cyclooxygenase-1 (COX-1), its target protein. These results suggested that the position of PC groups realized hydrophilic polymer surface, while MPC based polymer was not able to supply the suitable environment for COX-1 to interact with Ibu.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume84
Issue number1
DOIs
Publication statusPublished - 2011 May 1

Keywords

  • Phosphorylcholine groups
  • Polymer surface properties
  • Protein capturing test

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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