Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of microbial community structure in landfill leachate

Miho Uchida, Haruna Hatayoshi, Aoi Syuku-nobe, Takefumi Shimoyama, Toru Nakayama, Akitsugu Okuwaki, Tokuzo Nishino, Hisashi Hemmi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The structures of microbial communities in water samples obtained from a landfill site that had been a source of environmental pollution by emitting hydrogen sulfide were elucidated using polymerase chain reaction-denaturing gradient gel electrophoresis. The microbial communities, which consisted of a limited number of major microorganisms, were stable for several months. Microorganisms capable of degrading such chemical compounds as 2-hydroxybenzothiazole and bisphenol A were observed in landfill leachate. Microorganisms responsible for the production of hydrogen sulfide were not the primary microbes detected, even in water samples obtained from the site of gas emission.

Original languageEnglish
Pages (from-to)1503-1508
Number of pages6
JournalJournal of Hazardous Materials
Volume164
Issue number2-3
DOIs
Publication statusPublished - 2009 May 30

Keywords

  • Hydrogen sulfide
  • Landfill
  • Leachate
  • Microbial community
  • PCR-DGGE

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

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