Polymer conjugation to Cu,Zn-SOD and suppression of hydroxyl radical generation on exposure to H2O2: Improved stability of SOD in vitro and in vivo

Yuichiro Kojima; Takaaki Akaike; Keizo Sato; Hiroshi Maeda; Takashi Hirano

doi:10.1177/088391159601100301

Polymer conjugation to Cu,Zn-SOD and suppression of hydroxyl radical generation on exposure to H₂O₂: Improved stability of SOD in vitro and in vivo

Yuichiro Kojima, Takaaki Akaike, Keizo Sato, Hiroshi Maeda, Takashi Hirano

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Highly reactive hydroxyl radical (·OH) is generated during reaction of Cu,Zn-SOD(SOD) with H₂O₂, its own enzymatic reaction product (SOD plus O₂ ^·-). During inactivation of Cu,Zn-SOD with H₂O₂, Cu²⁺ liberated from the SOD catalyzes the formation of ·OH from H₂O₂. The ·OH thus generated will react with α₁-protease inhibitor (α₁-PI) if it is available in the milieu. To circumvent ·OH generation, this paper prepared eight different polymer-SOD conjugates and compared their stability against H₂O₂ treatment. Furthermore, the effect of each polymer-SOD conjugate on α₁-PI in the presence of H₂O₂ was tested.

Original language	English
Pages (from-to)	169-190
Number of pages	22
Journal	Journal of Bioactive and Compatible Polymers
Volume	11
Issue number	3
DOIs	https://doi.org/10.1177/088391159601100301
Publication status	Published - 1996 Jan 1
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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Polymer conjugation to Cu,Zn-SOD and suppression of hydroxyl radical generation on exposure to H₂O₂ : Improved stability of SOD in vitro and in vivo. / Kojima, Yuichiro; Akaike, Takaaki; Sato, Keizo; Maeda, Hiroshi; Hirano, Takashi.

In: Journal of Bioactive and Compatible Polymers, Vol. 11, No. 3, 01.01.1996, p. 169-190.

Research output: Contribution to journal › Article › peer-review

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