Polymer conjugation to Cu,Zn-SOD and suppression of hydroxyl radical generation on exposure to H2O2: Improved stability of SOD in vitro and in vivo

Yuichiro Kojima, Takaaki Akaike, Keizo Sato, Hiroshi Maeda, Takashi Hirano

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Highly reactive hydroxyl radical (·OH) is generated during reaction of Cu,Zn-SOD(SOD) with H2O2, its own enzymatic reaction product (SOD plus O2 ·-). During inactivation of Cu,Zn-SOD with H2O2, Cu2+ liberated from the SOD catalyzes the formation of ·OH from H2O2. The ·OH thus generated will react with α1-protease inhibitor (α1-PI) if it is available in the milieu. To circumvent ·OH generation, this paper prepared eight different polymer-SOD conjugates and compared their stability against H2O2 treatment. Furthermore, the effect of each polymer-SOD conjugate on α1-PI in the presence of H2O2 was tested.

Original languageEnglish
Pages (from-to)169-190
Number of pages22
JournalJournal of Bioactive and Compatible Polymers
Volume11
Issue number3
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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