TY - JOUR
T1 - Polymer conjugation to Cu,Zn-SOD and suppression of hydroxyl radical generation on exposure to H2O2
T2 - Improved stability of SOD in vitro and in vivo
AU - Kojima, Yuichiro
AU - Akaike, Takaaki
AU - Sato, Keizo
AU - Maeda, Hiroshi
AU - Hirano, Takashi
PY - 1996/1/1
Y1 - 1996/1/1
N2 - Highly reactive hydroxyl radical (·OH) is generated during reaction of Cu,Zn-SOD(SOD) with H2O2, its own enzymatic reaction product (SOD plus O2
·-). During inactivation of Cu,Zn-SOD with H2O2, Cu2+ liberated from the SOD catalyzes the formation of ·OH from H2O2. The ·OH thus generated will react with α1-protease inhibitor (α1-PI) if it is available in the milieu. To circumvent ·OH generation, this paper prepared eight different polymer-SOD conjugates and compared their stability against H2O2 treatment. Furthermore, the effect of each polymer-SOD conjugate on α1-PI in the presence of H2O2 was tested.
AB - Highly reactive hydroxyl radical (·OH) is generated during reaction of Cu,Zn-SOD(SOD) with H2O2, its own enzymatic reaction product (SOD plus O2
·-). During inactivation of Cu,Zn-SOD with H2O2, Cu2+ liberated from the SOD catalyzes the formation of ·OH from H2O2. The ·OH thus generated will react with α1-protease inhibitor (α1-PI) if it is available in the milieu. To circumvent ·OH generation, this paper prepared eight different polymer-SOD conjugates and compared their stability against H2O2 treatment. Furthermore, the effect of each polymer-SOD conjugate on α1-PI in the presence of H2O2 was tested.
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U2 - 10.1177/088391159601100301
DO - 10.1177/088391159601100301
M3 - Article
AN - SCOPUS:0030199059
VL - 11
SP - 169
EP - 190
JO - Journal of Bioactive and Compatible Polymers
JF - Journal of Bioactive and Compatible Polymers
SN - 0883-9115
IS - 3
ER -