The objective of this study was to synthesize and characterize novel threedimensional porous scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/nano-TiO2-particle composite microspheres for potential bone repair applications. The introduction of TiO2 component has been proven capable of largely enhancing mechanical properties of PLGA/ TiO 2 microsphere-sintered scaffold ("PLGA/TiO2- SMS"). In addition, composite nano-TiO2 additives are capable of inducing an increased arrest of adhesive proteins from the environment, which benefits cell attachment onto the scaffolds. Osteoblast proliferation and maturation were evaluated by MTT assay, alkaline phosphatase (ALP) activity, and bony calcification assay. The results indicate that osteoblasts cultured on the composite scaffolds with different TiO2 content (0, 0.1, and 0.3 g/1 g PLGA) display increased cell proliferation compared with pure PLGA scaffold. When cultured on composite scaffolds, osteoblasts also exhibit significantly enhanced ALP activity and higher calcium secretion, with respect to those on the pure PLGA scaffolds. Taken together, PLGA/TiO2-SMSs deserve attention utilizing for potential bone-repairing therapeutics.
|Number of pages||9|
|Journal||Journal of Biomedical Materials Research - Part B Applied Biomaterials|
|Publication status||Published - 2010 Apr|
- Bone repair
- TiO nanoparticles
ASJC Scopus subject areas
- Biomedical Engineering