Abstract
Lipids extracted from the shiitake mushroom Lentinus edodes contain dolichols composed of 15 up to 19 isoprene units with Dol-17 as the dominating prenologue. Identification of dolichols was achieved by the application of 2D-TLC, HPLC and electrospray ionization mass spectrometry. Additionally a family of polyprenols (α-unsaturated counterparts) with the same chain-length was also detected. Dolichols comprised approximately 0.002% of the fresh weight of the mushroom. Dolichols accompanied by traces of polyprenols are for the first time found in the mushroom tissue.
Original language | English |
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Pages (from-to) | 109-115 |
Number of pages | 7 |
Journal | Chemistry and Physics of Lipids |
Volume | 130 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2004 Jul |
Keywords
- Dolichol
- Electrospray mass spectrometry
- Lentinus edodes
- Polyisoprenoid
- Two-dimensional thin layer chromatography
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Organic Chemistry
- Cell Biology