Polyglutamine-containing microglia leads to disturbed differentiation and neurite retraction of neuron-like cells

Ryuji Owada, Saaya Awata, Kazutomo Suzue, Hiroyasu Kanetaka, Yohei Kakuta, Kazuhiro Nakamura

Research output: Contribution to journalArticlepeer-review

Abstract

Expanded polyglutamine-containing proteins in neurons intrinsically contributes to neuronal dysfunctions and neuronal cell death in polyglutamine (polyQ) diseases. In addition, an expanded polyQ-containing protein in microglia also leads to apoptosis of neurons. However, detailed morphological analysis of neurons exposed to conditioned medium (CM) derived from polyQ-containing microglia has not been essentially carried out. Here, we introduced aggregated peptide with 69 glutamine repeat (69Q) into BV2 microglial cells. The 69Q-containing BV2 cells showed shorter branches. The CM from 69Q-containing microglia (69Q-CM) induced neurite retraction and fewer number of branch point of neurites of differentiated PC12 cells. Likewise, the 69Q-CM induces disturbed differentiation of PC12 cells with shorter total length of neurites and fewer number of branch point of neurites. Thus, the factor(s) released from polyQ-containing microglia affect both differentiation and degeneration of neuron-like cells.

Original languageEnglish
Article numbere04851
JournalHeliyon
Volume6
Issue number9
DOIs
Publication statusPublished - 2020 Sep

Keywords

  • Cell culture
  • Cell differentiation
  • Molecular neuroscience
  • Neurite
  • Neuroanatomy
  • Neuronal death
  • Polyglutamine

ASJC Scopus subject areas

  • General

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