Polyelectrolyte multilayer microcapsules containing fluorescein isothiocyanate-concanavalin a/glycogen conjugates for fluorometric determination of sugars

Katsuhiko Sato, Yoshihiro Endo, Jun Ichi Anzai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Polyelectrolyte multilayer microcapsules were prepared by a layer-by-layer deposition of poly(ethyleneimine) (PEI) or poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS) on a calcium carbonate particle containing fluorescein isothiocyanate-concanavalin A (FITC-Con A)/glycogen conjugates. Fluorescence microscope and scanning electron microscope observations revealed that the microcapsules thus prepared are spherical with a diameter of 3-5 μm. The fluorescence intensity of the microcapsules was enhanced upon addition of sugars, depending on the type of sugar and its concentration. The enhanced fluorescence intensity was rationalized by sugar-induced decomposition of the FITC-Con A/glycogen conjugates and resulting dequenching of the fluorescence of FITC-Con A. The capsule can be used repeatedly a few times after washing in buffer solution, although FITC-Con A leaked out of the capsules gradually.

Original languageEnglish
Pages (from-to)203-213
Number of pages11
JournalSensors and Materials
Volume19
Issue number4
Publication statusPublished - 2007 Aug 9

Keywords

  • Con A/glycogen conjugate
  • Concanavalin A
  • D-glucose detection
  • Glycogen
  • Microcapsule

ASJC Scopus subject areas

  • Instrumentation
  • Materials Science(all)

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