Polydimethylsiloxane microfluidic films for in vitro engineering of small-scale neuronal networks

Research output: Contribution to journalArticlepeer-review

Abstract

Polydimethylsiloxane microfluidic devices have become standard tools in cell engineering research. However, through-holes where cells access the microchannels are usually fabricated manually using biopsy punches, making it difficult to create a large array of sub-mm sized through-holes. Here, we present a fabrication process for a thin-film microfluidic device containing an array of through-holes, which are as small as 100 μm by 100 μm and span 10 mm by 10 mm. A proof-of-concept application of the device to neuronal patterning experiments shows that spatially complex network dynamics emerge when a non-random connectivity is imposed to cultured neuronal networks. We also demonstrate that the coupling strengths between neuronal modules, a major factor that defines the global network dynamics, can be effectively modulated by varying the microchannel widths. This work opens a new application of microfluidic devices to multicellular systems comprised of several tens to hundreds of neurons.

Original languageEnglish
Article number117001
JournalJapanese journal of applied physics
Volume59
Issue number11
DOIs
Publication statusPublished - 2020 Nov

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Polydimethylsiloxane microfluidic films for in vitro engineering of small-scale neuronal networks'. Together they form a unique fingerprint.

Cite this