Polyamine oxidase 7 is a terminal catabolism-type enzyme in oryza sativa and is specifically expressed in anthers

Taibo Liu, Dong Wook Kim, Masaru Niitsu, Shunsuke Maeda, Masao Watanabe, Yoshiyuki Kamio, Thomas Berberich, Tomonobu Kusano

    Research output: Contribution to journalArticlepeer-review

    38 Citations (Scopus)

    Abstract

    Polyamine oxidase (PAO), which requires FAD as a cofactor, functions in polyamine catabolism. Plant PAOs are classified into two groups based on their reaction modes. The terminal catabolism (TC) reaction always produces 1,3-diaminopropane (DAP), H2O2, and the respective aldehydes, while the back-conversion (BC) reaction produces spermidine (Spd) from tetraamines, spermine (Spm) and thermospermine (T-Spm) and/or putrescine from Spd, along with 3-aminopropanal and H2O2. The Oryza sativa genome contains seven PAO-encoded genes termed OsPAO1-OsPAO7. To date, we have characterized four OsPAO genes. The products of these genes, i.e. OsPAO1, OsPAO3, OsPAO4 and OsPAO5, catalyze BC-type reactions. Whereas OsPAO1 remains in the cytoplasm, the other three PAOs localize to peroxisomes. Here, we examined OsPAO7 and its gene product. OsPAO7 shows high identity to maize ZmPAO1, the best characterized plant PAO having TC-type activity. OsPAO7 seems to remain in a peripheral layer of the plant cell with the aid of its predicted signal peptide and transmembrane domain. Recombinant OsPAO7 prefers Spm and Spd as substrates, and it produces DAP from both substrates in a time-dependent manner, indicating that OsPAO7 is the first TC-type enzyme identified in O. sativa. The results clearly show that two types of PAOs co-exist in O. sativa. Furthermore, OsPAO7 is specifically expressed in anthers, with an expressional peak at the bicellular pollen stage. The physiological function of OsPAO7 in anthers is discussed.

    Original languageEnglish
    Pages (from-to)1110-1122
    Number of pages13
    JournalPlant and Cell Physiology
    Volume55
    Issue number6
    DOIs
    Publication statusPublished - 2014 Jun

    Keywords

    • Anther
    • Oryza sativa
    • Polyamine
    • Polyamine oxidase
    • Terminal catabolism

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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