Poly lactic acid-caprolactone copolymer tube with a denatured skeletal muscle segment inside as a guide for peripheral nerve regeneration: A morphological and electrophysiological evaluation of the regenerated nerves

Nurru L. Mligiliche, Yasuhiko Tabata, Masaaki Kitada, Katsuaki Endoh, Keiko Okamato, Etsuko Fujimoto, Chizuka Ide

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A biodegradable copolymer of poly L-lactic acid and E-caprolactone (PLAC) was manufactured into a tube, in which a denatured skeletal muscle segment was placed longitudinally. This model tube was implanted as a guide to promote nerve regeneration across a 5 cm gap in the rabbit sciatic nerve. Five months after implantation, good nerve regeneration was found throughout the graft and in the distal host nerve. The population (29.6/16 × 102 μm2) of regenerated nerves in the graft was higher than that of the contralateral normal sciatic nerve (18.0/16 × 102 μm2). Regenerated nerve fibers extended to the distal host nerve. The number of myelinated fibers was 13.7/16 × 102 μm2 at a level 1.5 cm from the distal suture. The diameters (below 2 μm) of most regenerated myelinated nerves in the graft and in the distal host nerve were much smaller than those (6-8 μm) of normal nerves. Electrophysiological evaluation showed that the hindlimb muscle (gastrocnemius) was innervated by motor nerves in all animals 5 months after implantation. These results indicate that the PLAC tube with a denatured muscle segment inside provided good conditions for nerve fiber regrowth. The PLAC tube is thought to protect the denatured muscle segment from rapid dissociation in the host tissue.

Original languageEnglish
Pages (from-to)156-161
Number of pages6
JournalAnatomical Science International
Volume78
Issue number3
Publication statusPublished - 2003 Sep 1

Keywords

  • Biodegradable tube
  • Caprolactone copolymer
  • Denatured skeletal muscle
  • Grafting
  • Peripheral nerve regeneration
  • Poly L-lactic acid-ε

ASJC Scopus subject areas

  • Anatomy

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