Point mutation of c-k-ras oncogene at codon 12 in mucin-producing cystic tumor of the pancreas

Kennichi Satoh, Takashi Sawal, Tooru Shimosegawa, Masaru Koizumi, Tadashi Yamazaki, Fukuji Mochizuki, Takayoshi Toyota

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6 Citations (Scopus)

Abstract

The point mutation of c-K-ras oncogene at codon 12 was investigated in 15 cases of mucin-producing cystic tumor of the pancreas by a modified polymerase chain reaction (PCR) method. Three percent formalin-fixed and paraffin embedded materials were employed for the present investigation. These fifteen cases were histopathologically classified into five categories (from group I to V) according to Kozuka’s classification which was based on cell atypia of the tumor cells; 5 cases were judged as group V, 4 cases as group IV, and 6 cases as group III. The frequency of point mutation was 5/5 in group V, 3/4 in group IV, and 2/6 in group III. The frequency of point mutation was correlated with morphologically determined cell atypia. Among group III, the point mutation was noticed in the cases with larger tumor size. From genetical point of view, the results indicated more malignant biological feature of mucin-producing cystic tumor of the pancreas than has generally been accepted.

Original languageEnglish
Pages (from-to)2795-2803
Number of pages9
JournalNippon Shokakibyo Gakkai Zasshi
Volume88
Issue number11
DOIs
Publication statusPublished - 1991 Jan 1

Keywords

  • K-ras gene
  • PCR

ASJC Scopus subject areas

  • Gastroenterology

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    Satoh, K., Sawal, T., Shimosegawa, T., Koizumi, M., Yamazaki, T., Mochizuki, F., & Toyota, T. (1991). Point mutation of c-k-ras oncogene at codon 12 in mucin-producing cystic tumor of the pancreas. Nippon Shokakibyo Gakkai Zasshi, 88(11), 2795-2803. https://doi.org/10.11405/nisshoshi1964.88.2795