PMab-44 Detects Bovine Podoplanin in Immunohistochemistry

Ryusuke Honma, Satoshi Ogasawara, Mika K. Kaneko, Yuki Fujii, Hiroharu Oki, Takuro Nakamura, Michiaki Takagi, Satoru Konnai, Yukinari Kato

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Podoplanin (PDPN)/Aggrus is a type I transmembrane O-glycoprotein, which is expressed in several normal tissues including podocytes of kidney and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelet; however, only bovine PDPN (bovPDPN) does not possess the platelet-aggregating activity. Although many monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, and rabbit PDPN have been established, anti-bovPDPN mAbs have not been developed. In this study, we immunized mice with the recombinant proteins of bovPDPN and developed anti-bovPDPN mAbs, which are useful in immunohistochemical analysis. One of the clones, PMab-44, is useful for detecting podocytes and lymphatic endothelial cells in normal bovine tissues. PMab-44 also detected bovPDPN specifically in flow cytometry. PMab-44 is expected to be useful for investigating the function of bovPDPN.

Original languageEnglish
Pages (from-to)186-190
Number of pages5
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume35
Issue number4
DOIs
Publication statusPublished - 2016 Aug

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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