PMab-247 Detects Bear Podoplanin in Immunohistochemical Analysis

Junko Takei, Yoshikazu Furusawa, Shinji Yamada, Takuro Nakamura, Yusuke Sayama, Masato Sano, Satoru Konnai, Atsushi Kobayashi, Hiroyuki Harada, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Podoplanin (PDPN) is utilized as a specific marker of type I alveolar cells of lung and lymphatic endothelial cells of every tissue. Therefore, sensitive and specific monoclonal antibodies detecting PDPN are necessary for immunohistochemical analyses, especially using formalin-fixed paraffin-embedded tissues. Recently, we developed an anti-bear PDPN (bPDPN) mAb, PMab-247, which is useful for Western blot, flow cytometry, and immunohistochemical analyses. In this study, immunohistochemical analyses showed that PMab-247 strongly detected bPDPN, which is expressed in type I alveolar cells and lymphatic endothelial cells of bear lung and podocytes of bear kidney. These findings suggest that PMab-247 could be useful for pathophysiological analyses using immunohistochemistry.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number4
DOIs
Publication statusPublished - 2019 Aug

Keywords

  • PDPN
  • PMab-247
  • bear podoplanin
  • lymphatic endothelial cells
  • type I alveolar cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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