PMab-241 specifically detects bear podoplanin of lymphatic endothelial cells in the lung of brown bear

Junko Takei, Shinji Yamada, Satoru Konnai, Tsuyoshi Ishinazaka, Michito Shimozuru, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Podoplanin (PDPN)/T1alpha is utilized as a specific marker of lymphatic endothelial cells or type I alveolar cells of lung. Therefore, sensitive and specific monoclonal antibodies (mAbs) detecting PDPN are necessary for immunohistochemical analyses, especially using formalin-fixed paraffin-embedded tissues. Recently, we developed an anti-bear PDPN (bPDPN) mAb, PMab-247, which is useful for immunohistochemical analyses to detect both lymphatic endothelial cells and type I alveolar cells of lung. However, it is difficult to distinguish lymphatic endothelial cells from type I alveolar cells in the bear lung. In this study, we showed that a novel anti-bPDPN mAb, PMab-241 stained only lymphatic endothelial cells, not type I alveolar cells of the lung in immunohistochemical analyses. These findings suggest that PMab-241 could be useful for staining lymphatic endothelial cells specifically in the bear lung tissues.

Original languageEnglish
Pages (from-to)282-284
Number of pages3
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number6
DOIs
Publication statusPublished - 2019 Dec

Keywords

  • PDPN
  • PMab-241
  • bear podoplanin
  • lymphatic endothelial cells
  • type I alveolar cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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