PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin

Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Masato Sano, Hiroyuki Harada, Masato Fukui, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG2a, kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting. Furthermore, PMab-219 strongly stained CHO/horPDPN via immunohistochemistry. These findings suggest that PMab-219 is useful for investigating the function of horPDPN.

Original languageEnglish
Article number100616
JournalBiochemistry and Biophysics Reports
Volume18
DOIs
Publication statusPublished - 2019 Jul

Keywords

  • Horse podoplanin
  • PDPN
  • PMab-219

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin'. Together they form a unique fingerprint.

Cite this