PMab-210: A Monoclonal Antibody Against Pig Podoplanin

Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Masato Fukui, Hiroyuki Harada, Takuya Mizuno, Yusuke Sakai, Masaki Takasu, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Podoplanin (PDPN) is a type I transmembrane glycoprotein that is expressed in normal tissues, including renal corpuscles and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPNs have already been established; however, antipig PDPN (pPDPN) mAbs have not. We therefore immunized mice with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and screened hybridomas, which are producing anti-pPDPN mAbs. One of mAbs, PMab-210 (an IgG 1 , kappa), was able to specifically detect CHO/pPDPN cells by flow cytometry and detect pPDPN by Western blot analysis. Furthermore, PMab-210 strongly stained type I lung alveolar cells and weakly stained renal corpuscles by immunohistochemistry. PMab-210 is expected to be useful in investigating the function of pPDPN.

Original languageEnglish
Pages (from-to)30-36
Number of pages7
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number1
DOIs
Publication statusPublished - 2019 Feb

Keywords

  • PDPN
  • PMab-210
  • Pig podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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