Platelet-activating factor in the inflammatory exudate in the anaphylactic phase of allergic inflammation in rats

Masako Watanabe, Kazuo Ohuchi, Atsuhiro Sugidachi, Noriyasu Hirasawa, Yasuhiro Hayashi, Susumu Tsurufuji

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Using a model of allergic inflammation of air pouch type in rats, the platelet-activating factor (PAF) in the pouch fluid in the anaphylactic phase was analyzed. Anaphylactic reaction was induced by injecting an antigen (azobenzene-arsonate-conjugated acetyl bovine serum albumin) solution into a subcutaneous air pouch preformed on the dorsum of immunized rats. The pouch fluid was collected 30 min after the antigenic challenge, and chloroform extract was subjected to normal phase high-performance liquid chromatography to isolate two fractions, PAF and lyso-PAF. In the pouch fluid, however, there was little activity of PAF as examined by the aggregation of guinea pig platelets. The lyso-PAF fraction obtained was acetylated to PAF chemically with pyridine and acetic anhydride. This acetylated lyso-PAF fraction induced the aggregation of guinea pig platelets, which was inhibited dose-dependently by a PAF antagonist, CV-3988. The amount of lyso-PAF in the pouch fluid of the immunized group in the anaphylactic phase was significantly higher than that of the nonimmunized group. When (3H-)PAF was incubated with the supernatant fraction of the pouch fluid it was metabolized into lyso-PAF time-dependently. The significance of the higher level of lyso-PAF in the pouch fluid in the anaphylactic phase of allergic inflammation is discussed.

Original languageEnglish
Pages (from-to)396-403
Number of pages8
JournalInternational archives of allergy and immunology
Volume84
Issue number4
DOIs
Publication statusPublished - 1987 Jan 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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