Platelet-activating factor acetylhydrolase isoforms I and II in human uterus. Comparisons with pregnant uterus and myoma

K. Yasuda, T. Okumura, H. Okada, T. Nakajima, J. Aoki, H. Arai, K. Inoue, M. Nishizawa, S. Ito, H. Kanzaki

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The concentrations of platelet-activating factor (PAF) that possesses the ability to stimulate myometrial contraction are partially regulated by intracellular type of platelet-activating factor acetylhydrolase (PAF-AH) in many tissues. Tissue cytosol contains at least two intracellular PAF-AH, isoforms I and II. To examine the relationship between the activity and isoforms of intracellular PAF-AH in human uterine myometrium and myoma, we assayed the PAF-AH activity and identified the PAF-AH isoforms I and II by Western blot analysis. The intense bands of the α2 and β subunits of PAF-AH isoform I were detected in nonpregnant uterus; however, the specific bands of the α1 subunit of PAF-AH isoform I and the PAF-AH isoform II were extremely weak. The levels of the α2 and β subunits and PAF-AH activity in pregnant uterus (37-39 wk gestation) were significantly lower than those in nonpregnant uterus. On the other hand, the level of β subunit and the PAF-AH activity in myoma were significantly higher than those in nonpregnant uterus. No significant difference was found in the expression of the PAF-AH isoform II among three tissues. These results indicate that the change in the PAF-AH activity observed in pregnant uterus and myoma are due to the lower or higher protein expression of the PAF-AH isoform I, especially the α2 and/or β subunits. The decrease of the uterine PAF-AH activity in the late stage of pregnancy may facilitate the action of PAF to stimulate myoinertial contraction.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalBiology of Reproduction
Volume64
Issue number1
DOIs
Publication statusPublished - 2001

Keywords

  • Parturition
  • Pregnancy
  • Uterus

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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