TY - JOUR
T1 - Plastid proteins crucial for symbiotic fungal and bacterial entry into plant roots
AU - Imaizumi-Anraku, Haruko
AU - Takeda, Naoya
AU - Charpentier, Myriam
AU - Perry, Jillian
AU - Miwa, Hiroki
AU - Umehara, Yosuke
AU - Kouchi, Hiroshi
AU - Murakami, Yasuhiro
AU - Mulder, Lonneke
AU - Vickers, Kate
AU - Pike, Jodie
AU - Downie, J. Allan
AU - Wang, Trevor
AU - Sato, Shusei
AU - Asamizu, Erika
AU - Tabata, Satoshi
AU - Yoshikawa, Makoto
AU - Murooka, Yoshikatsu
AU - Wu, Guo Jiang
AU - Kawaguchi, Masayoshi
AU - Kawasaki, Shinji
AU - Parniske, Martin
AU - Hayashi, Makoto
N1 - Funding Information:
This work was supported by the NIH.
Funding Information:
Acknowledgements We thank K. Szczyglowski, J. Webb and J. Stougaard for providing mutant seeds; M. Hayashi for help with mapping; T. Kojima and R. Ohtomo for mycorrhiza analysis; Y. Niwa for providing pUC18-CaMV35S-sGFP (S65T)-nos vector; G. Oldroyd and J. Sun for help with Ca-spiking assays; J. Krüger and B. B. H. Wulff for critical reading of the manuscript; J. Soll for providing the pea root transformation protocol before publication; and M. Durrant for help with modelling the CASTOR pore structure. Part of this work was supported by the fund of Promotion of Basic Research Activities for Innovative Biosciences (BRAIN), and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency. Research at the Sainsbury Laboratory is funded by the Gatsby Charitable Foundation.
PY - 2005/2/3
Y1 - 2005/2/3
N2 - The roots of most higher plants form arbuscular mycorrhiza, an ancient, phosphate-acquiring symbiosis with fungi, whereas only four related plant orders are able to engage in the evolutionary younger nitrogen-fixing root-nodule symbiosis with bacteria. Plant symbioses with bacteria and fungi require a set of common signal transduction components that redirect root cell development. Here we present two highly homologous genes from Lotus japonicus, CASTOR and POLLUX, that are indispensable for microbial admission into plant cells and act upstream of intracellular calcium spiking, one of the earliest plant responses to symbiotic stimulation. Surprisingly, both twin proteins are localized in the plastids of root cells, indicating a previously unrecognized role of this ancient endosymbiont in controlling intracellular symbioses that evolved more recently.
AB - The roots of most higher plants form arbuscular mycorrhiza, an ancient, phosphate-acquiring symbiosis with fungi, whereas only four related plant orders are able to engage in the evolutionary younger nitrogen-fixing root-nodule symbiosis with bacteria. Plant symbioses with bacteria and fungi require a set of common signal transduction components that redirect root cell development. Here we present two highly homologous genes from Lotus japonicus, CASTOR and POLLUX, that are indispensable for microbial admission into plant cells and act upstream of intracellular calcium spiking, one of the earliest plant responses to symbiotic stimulation. Surprisingly, both twin proteins are localized in the plastids of root cells, indicating a previously unrecognized role of this ancient endosymbiont in controlling intracellular symbioses that evolved more recently.
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U2 - 10.1038/nature03237
DO - 10.1038/nature03237
M3 - Article
C2 - 15616514
AN - SCOPUS:13444301253
VL - 433
SP - 527
EP - 531
JO - Nature
JF - Nature
SN - 0028-0836
IS - 7025
ER -