Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing)

Rie Terada, Junko Kyozuka, Soryu Nishibayashi, Ko Shimamoto

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Somatic hybridization of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola), a close relative of barnyard millet, was attempted using electrofusion and a new culture method developed for rice protoplasts (Kyozuka et al. 1987) to incorporate some of the agronomically important characters of the latter species into rice. Selection of hybrids was based on inactivation of rice protoplasts by iodoacetamide and the inability of barnyard grass protoplasts to divide. A total of 166 calli were identified as hybrids by isozyme and chromosome analyses. Hybrid calli were highly morphogenic, and 44 shoots were obtained. Most of them, however, were abnormal, and nine grew to plantlets whose morphology was distinct from that of either parent. Our study clearly demonstrates the totipotency of protoplasts in graminaceous monocots.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalMGG Molecular & General Genetics
Volume210
Issue number1
DOIs
Publication statusPublished - 1987 Nov 1
Externally publishedYes

Keywords

  • Cell fusion
  • Gramineae
  • Intertribal hybrid
  • Oryza sativa
  • Protoplast

ASJC Scopus subject areas

  • Genetics

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