Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and in Bax-induced cell death

Chika Tateda, Koji Yamashita, Fumio Takahashi, Tomonobu Kusano, Yoshihiro Takahashi

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The voltage-dependent anion channel (VDAC) is a major outer mitochondrial membrane protein. It is well documented that VDAC plays an important role in apoptosis, a kind of programmed cell death, in mammalian systems. However, little is known about the role of the plant counterpart during the process of plant-specific cell death such as pathogen-induced hypersensitive response. To address this issue, we isolated three VDAC full-length cDNAs (NtVDAC1-3) from Nicotiana tabacum. The deduced products, NtVDACs, share 78-85% identity and retain the conserved eukaryotic mitochondrial porin signature distal to their C-terminal regions. Mitochondrial localization of three NtVDACs in plant cells was confirmed via a green fluorescent protein fusion method. Then, we addressed the main issue concerning pathogenesis relation. The N. benthamiana orthologues of NtVDACs were upregulated by challenge with the non-host pathogen Pseudomonas cichorii, but not after challenge with the virulent pathogen P. syringae pv. tabaci. Both the pharmaceutical inhibition of VDAC and silencing of NbVDACs genes compromised the non-host resistance against P. cichorii, suggesting the involvement of VDACs in defense against non-host pathogen. Involvement of NbVDACs in Bax-mediated cell death was also verified using a similar approach.

Original languageEnglish
Pages (from-to)41-51
Number of pages11
JournalPlant Cell Reports
Volume28
Issue number1
DOIs
Publication statusPublished - 2009 Jan
Externally publishedYes

Keywords

  • Bax
  • Mitochondrion
  • N. tabacum
  • Pathogen defense
  • VDAC

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Fingerprint

Dive into the research topics of 'Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and in Bax-induced cell death'. Together they form a unique fingerprint.

Cite this