Plant regeneration from protoplasts of indica rice: genotypic differences in culture response

J. Kyozuka, E. Otoo, K. Shimamoto

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

Fourteen varieties of indica rice (Oryza saliva L.) were examined for their capacity for plant regeneration from protoplasts using the nurse culture methods developed for japonica rice. Calli induced from germinating seeds were grouped into two types: type I, white and compact; type II, yellow and friable. In four varieties producing type II callus, colony formation (2%-4.5%) and plant regeneration (2%-35%) were observed. The inability to develop suspension cultures was a major obstacle in regenerating plants from protoplasts of the remaining rice varieties studied.

Original languageEnglish
Pages (from-to)887-890
Number of pages4
JournalTheoretical and Applied Genetics
Volume76
Issue number6
DOIs
Publication statusPublished - 1988 Dec
Externally publishedYes

Keywords

  • Cereals
  • Indica rice
  • Plant regeneration
  • Protoplast
  • Rice (Oryza sativa L.)

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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