Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris spp. pekinensis)

Takanori Sato, Takeshi Nishio, Masashi Hirai

    Research output: Contribution to journalArticlepeer-review

    63 Citations (Scopus)

    Abstract

    Isolated microspores of Chinese cabbage (Brassica campestris ssp. pekinensis) were incubated in modified NN medium containing 10% sucrose in darkness at 33°C for one day followed by culture at 25°C. After 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. Plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators.

    Original languageEnglish
    Pages (from-to)486-488
    Number of pages3
    JournalPlant Cell Reports
    Volume8
    Issue number8
    DOIs
    Publication statusPublished - 1989 Dec 1

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Plant Science

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