Plant regeneration from isolated microspore cultures of Chinese cabbage (Brassica campestris spp. pekinensis)

Takanori Sato, Takeshi Nishio, Masashi Hirai

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Isolated microspores of Chinese cabbage (Brassica campestris ssp. pekinensis) were incubated in modified NN medium containing 10% sucrose in darkness at 33°C for one day followed by culture at 25°C. After 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. Plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators.

Original languageEnglish
Pages (from-to)486-488
Number of pages3
JournalPlant Cell Reports
Volume8
Issue number8
DOIs
Publication statusPublished - 1989 Dec 1

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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