Placental expression of lysophosphatidic acid receptors in normal pregnancy and preeclampsia

Tatsuya Fujii, Takeshi Nagamatsu, Danny J. Schust, Mayuko Ichikawa, Keiichi Kumasawa, Shinichiro Yabe, Takayuki Iriyama, Yasushi Hirota, Yutaka Osuga, Junken Aoki, Yutaka Yatomi, Tomoyuki Fujii

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Problem: Recent advances in lipid research have revealed that impairments in lipid mediator signaling can be involved in the pathoetiology of a variety of diseases. We previously reported aberrant expression of autotaxin, a key enzyme for lysophosphatidic acid (LPA) production, in placentas from women with preeclampsia. The present study aimed to further explore the involvement of LPA signaling in the pathoetiology of preeclampsia. Method of study: Term placentas were obtained from deliveries after uncomplicated pregnancy (n = 18) and those complicated by preeclampsia (n = 24). First-trimester placental tissues were collected after elective terminations of pregnancy (n = 20). Placental expression of the six identified LPARs (LPAR1-6) was analyzed at protein and mRNA levels. Results: In normal pregnancy, the mRNA expression levels of all LPARs except LPAR4 were significantly higher in term. Levels of mRNA encoding LPAR2-5 were significantly increased in preeclampsia placentas compared with those in the normal term placentas. Using Western immunoblotting, only LPAR3 was noted to be increased at the protein level in placentas from preeclamptic pregnancies. This was validated by immunohistochemistry. Conclusion: In summary, the placental expression of LPARs, particularly LPAR3, is enhanced in preeclampsia, suggesting that disturbances in placental LPA signaling may be involved in the pathogenesis of preeclampsia.

Original languageEnglish
Article numbere13176
JournalAmerican Journal of Reproductive Immunology
Volume82
Issue number5
DOIs
Publication statusPublished - 2019 Nov 1

Keywords

  • human
  • lysophosphatidic acid receptor
  • placenta
  • preeclampsia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

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