PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription

Yuya Kamano, Makio Saeki, Hiroshi Egusa, Yoshito Kakihara, Walid A. Houry, Hirofumi Yatani, Yoshinori Kamisaki

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

PIH1D1 is the defining component of the R2TP complex. Recently, R2TP has been reported to stabilize mTOR (mammalian target of rapamycin), an important regulator of cell growth and protein synthesis. Two complexes of mTOR, mTORC1 and mTORC2, have been identified. We demonstrate that immunoprecipitation (IP) of PIH1D1 results in the co-IP of Raptor (mTORC1 specific), but not Rictor (mTORC2 specific), and that knockdown of PIH1D1 decreases mTORC1 assembly, S6 kinase phosphorylation (indicator of mTORC1 activity), and rRNA transcription without affecting mTORC2 in human breast cancer MCF-7 cells. In addition, we provide evidence that PIH1D1 is overexpressed in various breast cancer cell lines. These findings collectively suggest that PIH1D1 may have an important role in mTORC1 regulation in breast cancers.

Original languageEnglish
Pages (from-to)3303-3308
Number of pages6
JournalFEBS Letters
Volume587
Issue number20
DOIs
Publication statusPublished - 2013 Oct 11
Externally publishedYes

Keywords

  • Breast cancer
  • Monad
  • PIH1D1
  • R2TP
  • Ribosome
  • mTOR

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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