PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced apoptosis

Mika Inoue, Makio Saeki, Hiroshi Egusa, Hitoshi Niwa, Yoshinori Kamisaki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have previously reported that the two components of R2TP complex, RNA polymerase II-associated protein 3 (RPAP3), and Reptin, regulate apoptosis. Here we characterize another component of the complex, PIH1 domain containing protein 1 (PIH1D1). PIH1D1 interacts with both RPAP3 and Monad in HEK293 or U2OS cells. PIH1D1 transcripts were abundant in lung, leukocyte, and placenta. The reduction in endogenous PIH1D1 by siRNA enhanced apoptosis and caspase-3 activation induced by doxorubicin in U2OS cells. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway.

Original languageEnglish
Pages (from-to)340-344
Number of pages5
JournalBiochemical and biophysical research communications
Volume403
Issue number3-4
DOIs
Publication statusPublished - 2010 Dec 17
Externally publishedYes

Keywords

  • Apoptosis
  • Monad
  • PIH1D1
  • R2TP
  • RPAP3
  • Reptin
  • WDR92

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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