Piezoelectric actuator-based cell microstretch device with real-time imaging capability

Shinji Deguchi, Shoko Kudo, Tsubasa S. Matsui, Wenjing Huang, Masaaki Sato

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Cellular response to physical stretch has been extensively studied as a regulator of various physiological functions. For live cell microscopy combined with stretch experiments, cells are typically seeded on an extensible elastomer sheet. In this case, the position of the cells of interest tends to shift out of the field of view upon stretch, making real-time imaging of identical cells difficult. To circumvent this situation, here we describe a robust methodology in which these cell shifts are minimized. Cells are plated in a custom-designed stretch chamber with an elastomer sheet of a small cell culture area. The cell-supporting chamber is stretched on an inverted microscope by using a piezoelectric actuator that provides small, but precisely controlled displacements. Even under this small displacement within the filed of view, our device allows the cells to undergo physiologically relevant levels of stretch. Identical cells can thus be continuously observed during stretching, thereby potentially enabling imaging of stretch-triggered fast dynamics.

Original languageEnglish
Article number067110
JournalAIP Advances
Volume5
Issue number6
DOIs
Publication statusPublished - 2015 Jun 1

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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