PICK1 binds to calcineurin B and modulates the NFAT activity in PC12 cells

Tsutomu Iida, Hiroshi Egusa, Makio Saeki, Hirofumi Yatani, Yoshinori Kamisaki

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In the central nervous system, calcineurin has been implicated in a number of Ca2+-sensitive pathways, including the regulation of neurotransmitter release and modulation of synaptic plasticity. PDZ domain-containing proteins also play an important role in the targeting and clustering of synaptic proteins. Using a yeast two-hybrid screen, we herein identified the PDZ domain-containing protein PICK1 as a specific interactor of calcineurin B. The interaction of calcineurin B and PICK1 was confirmed by GST pull-down assay in HEK293 cells and immunoprecipitation using rat brain lysate. Calcineurin B contains the consensus C-terminal peptide sequence required for interacting with the PDZ domain. The deletion of this sequence was sufficient to abolish the interaction between calcineurin B and PICK1. In addition, the knockdown of PICK1 by RNA interference inhibited the calcineurin-dependent activation of NFAT in PC12 cells. These results suggest that PICK1 may be a positive regulator of calcineurin in the central nervous system.

Original languageEnglish
Pages (from-to)655-659
Number of pages5
JournalBiochemical and biophysical research communications
Volume375
Issue number4
DOIs
Publication statusPublished - 2008 Oct 31
Externally publishedYes

Keywords

  • Calcineurin B
  • NFAT
  • PDZ domain
  • PICK1
  • Yeast two-hybrid screening

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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