Physiology: Immunohistochemical distribution of progesterone, androgen and oestrogen receptors in the human ovary during the menstrual cycle: Relationship to expression of steroidogenic enzymes

Takashi Suzuki, Hironobu Sasano, Noriko Kimura, Midori Tamura, Takao Fukaya, Akira Yajima, Hiroshi Nagura

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

In order to characterize immunohistochemically the possible in-situ effects of gonadal steroid hormones in the human ovary during the menstrual cycle, we immunolocalized progesterone (PR), androgen (AR) and oestrogen (ER) receptors in 50 normal cycling human ovaries, and examined the relationship between these findings and the cellular localization of steroidogenic enzymes including cytochrome P-450 cholesterol side-chain cleavage (P-450scc) enzyme, 3β-hydroxysteroid dehydrogenase (3βHSD), cytochrome P-450 17α-hydroxylase (P-450c17) and cytochrome P-450 aromatase (P-450arom). A large number of stromal cells were positive for AR, regardless of the distance from a follicle. No steroidogenic enzymes were observed in the stromal cells. In the pre-antral follicle, AR was observed in the theca cells. P-450scc, 3βHSD and P-450c17 were sporadically expressed in the theca cells in relatively large-sized pre-antral follicles. ER was positive in the granulosa cells only in the P-450arom-positive antral or pre-ovulatory follicle, which is likely to be a selected follicle. In the corpus luteum, in the period from ovulation to the mid-secretory phase, PR immunoreactivity was observed in a large number of both the luteinized granulosa and the theca cells. All steroidogenic enzymes were observed in all corpora lutea, but ER was negative in any corpus luteum. In the atretic follicle, AR was present in the theca interna cells. P-450scc, 3βHSD and P-450c17 were observed in the theca interna cells in some atretic follicles. These immunohistochemical findings demonstrated that steroid hormones locally produced may have important roles, as local regulators, in the human ovary during the menstrual cycle

Original languageEnglish
Pages (from-to)1589-1595
Number of pages7
JournalHuman Reproduction
Volume9
Issue number9
DOIs
Publication statusPublished - 1994 Sep

Keywords

  • Androgen
  • Immunohistochemistry
  • Oestrogen
  • Progesterone
  • Receptor

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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