Physical distances between S-locus genes in various S haplotypes of Brassica rapa and B. oloracea

G. Suzuki, M. Watanabe, T. Nishio

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

In Brassica, self-incompatibility genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are located in the S-locus complex region with several other S-linked genes. The S locus is a highly polymorphic region: polymorphism has been observed not only in sequences of SLG and SRK but also in the location of the S-locus genes. In order to compare the physical location of the S-locus genes in various S haplotypes, we used six class-I S haplotypes of B. rapa and seven class-I S haplotypes of B. oleracea in this study. DNA gel blot analysis using pulsed-field gel electrophoresis (PFGE) showed that the physical distances between SLG and SRK in B. rapa are significantly shorter than those in B. oleracea and that the sizes of MluI and BssHII fragments harboring SLG and SRK are less variable within B. rapa than within B. oleracea. We concluded that several large genomic fragments might have been inserted into the S-locus region of B. oleracea after allelic differentiation of S-locus genes.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalTheoretical and Applied Genetics
Volume101
Issue number1-2
DOIs
Publication statusPublished - 2000 Jan 1

Keywords

  • B. oleracea
  • Brassica rapa (syn. campestris)
  • PFGE
  • S-locus
  • Self- incompatibility

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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