TY - JOUR
T1 - Phylogenetic and expression analysis of lamprey toll-like receptors
AU - Kasamatsu, Jun
AU - Oshiumi, Hiroyuki
AU - Matsumoto, Misako
AU - Kasahara, Masanori
AU - Seya, Tsukasa
N1 - Funding Information:
We are grateful to our laboratory members for invaluable discussions. This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, and Culture (Specified Project for Advanced Research) of Japan and in part by the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases, MEXT . The supports by the Mitsubishi Foundation (TS) , Takeda Foundation (TS) and Uehara memorial Foundation (MM) were gratefully acknowledged.
PY - 2010/8
Y1 - 2010/8
N2 - Toll-like receptors (TLRs) have been identified as pivotal sensors recognizing microbial pattern molecules in vertebrates. Whole genome analysis of the teleost. Takifugu rubripes supports the existence of a fundamental family of TLR genes in fish. However, the role of the innate immune system in the context of raising acquired immunity in jawless fish remains unclear. In this study, we annotated 16 lamprey TLR genes predicted from the latest genome assembly of lamprey on the basis of homology, and identified their cDNAs from Japanese lamprey, Lethenteron japonicum. Phylogenetic analyses indicated that the repertoire of lamprey TLRs consisted of both fish (F)- and mammalian (M)-type TLRs, and it was also demonstrated that lamprey TLRs are constitutively expressed in various organs. Our results suggest that lampreys protect against microorganisms using the innate system consisting of a similar set of M- and F-type TLRs, despite possessing a unique acquired immune system. In addition, type I interferon (IFN), interferon regulatory factor (IRF)- 3, and IRF7 were not identified in the lamprey genome although TLR adaptor and signal transduction genes were highly conserved upstream of (IRF)-3/7 and type I IFN in most vertebrates. This is the first report to describe the TLR repertoire and IFN system in one of the most primitive vertebrates, the lamprey.
AB - Toll-like receptors (TLRs) have been identified as pivotal sensors recognizing microbial pattern molecules in vertebrates. Whole genome analysis of the teleost. Takifugu rubripes supports the existence of a fundamental family of TLR genes in fish. However, the role of the innate immune system in the context of raising acquired immunity in jawless fish remains unclear. In this study, we annotated 16 lamprey TLR genes predicted from the latest genome assembly of lamprey on the basis of homology, and identified their cDNAs from Japanese lamprey, Lethenteron japonicum. Phylogenetic analyses indicated that the repertoire of lamprey TLRs consisted of both fish (F)- and mammalian (M)-type TLRs, and it was also demonstrated that lamprey TLRs are constitutively expressed in various organs. Our results suggest that lampreys protect against microorganisms using the innate system consisting of a similar set of M- and F-type TLRs, despite possessing a unique acquired immune system. In addition, type I interferon (IFN), interferon regulatory factor (IRF)- 3, and IRF7 were not identified in the lamprey genome although TLR adaptor and signal transduction genes were highly conserved upstream of (IRF)-3/7 and type I IFN in most vertebrates. This is the first report to describe the TLR repertoire and IFN system in one of the most primitive vertebrates, the lamprey.
KW - Evolution
KW - Innate immunity
KW - Interferon
KW - Lamprey
KW - Phylogeny
KW - Toll-like receptor
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U2 - 10.1016/j.dci.2010.03.004
DO - 10.1016/j.dci.2010.03.004
M3 - Article
C2 - 20363250
AN - SCOPUS:77952876470
VL - 34
SP - 855
EP - 865
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
IS - 8
ER -