Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

Ken Oikawa, Akihide Hibara, Takehiko Kitamori

Research output: Contribution to conferencePaperpeer-review

Abstract

This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10-6 absorbance while normal microscope can visualize 10-3 absorbance.

Original languageEnglish
Pages1277-1279
Number of pages3
Publication statusPublished - 2006 Jan 1
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

Keywords

  • Absorbance
  • Photothermal
  • Visualization

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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