Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

Ken Oikawa; Akihide Hibara; Takehiko Kitamori

Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

Ken Oikawa, Akihide Hibara, Takehiko Kitamori

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10^-6 absorbance while normal microscope can visualize 10^-3 absorbance.

Original language	English
Pages	1277-1279
Number of pages	3
Publication status	Published - 2006 Jan 1
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

Keywords

Absorbance
Photothermal
Visualization

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

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Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples. / Oikawa, Ken; Hibara, Akihide; Kitamori, Takehiko.

2006. 1277-1279 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

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abstract = "This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10-6 absorbance while normal microscope can visualize 10-3 absorbance.",

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N2 - This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10-6 absorbance while normal microscope can visualize 10-3 absorbance.

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KW - Photothermal

KW - Visualization

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