Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

Ken Oikawa; Akihide Hibara; Takehiko Kitamori

Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

Ken Oikawa, Akihide Hibara, Takehiko Kitamori

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10^-6 absorbance while normal microscope can visualize 10^-3 absorbance.

Original language	English
Pages	1277-1279
Number of pages	3
Publication status	Published - 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

Keywords

Absorbance
Photothermal
Visualization

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples. / Oikawa, Ken; Hibara, Akihide; Kitamori, Takehiko.

2006. 1277-1279 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

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title = "Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples",

abstract = "This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10-6 absorbance while normal microscope can visualize 10-3 absorbance.",

keywords = "Absorbance, Photothermal, Visualization",

author = "Ken Oikawa and Akihide Hibara and Takehiko Kitamori",

note = "Copyright: Copyright 2014 Elsevier B.V., All rights reserved.; 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

year = "2006",

language = "English",

pages = "1277--1279",

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T1 - Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

AU - Oikawa, Ken

AU - Hibara, Akihide

AU - Kitamori, Takehiko

PY - 2006

Y1 - 2006

N2 - This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10-6 absorbance while normal microscope can visualize 10-3 absorbance.

AB - This paper describes the development of a non-scanning photothermal imaging method by CCD-based optics. By utilizing visible excitation laser and lamp-based illumination with phase-contrast optics, image-acquiring time was 4 min for 100 μm × 100 μm areas. In the present stage, the system could detect 7.5 × 10-6 absorbance while normal microscope can visualize 10-3 absorbance.

KW - Absorbance

KW - Photothermal

KW - Visualization

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M3 - Paper

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T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Photothermally enhanced phase-contrast microscope for sensitive visualization of non-fluorescent samples

Abstract

Other

Keywords

ASJC Scopus subject areas

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