Photothermal imaging of skeletal muscle mitochondria

Toru Tomimatsu, Jun Miyazaki, Yutaka Kano, Takayoshi Kobayashi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The morphology and topology of mitochondria provide useful information about the physiological function of skeletal muscle. Previous studies of skeletal muscle mitochondria are based on observation with transmission, scanning electron microscopy or fluorescence microscopy. In contrast, photothermal (PT) microscopy has advantages over the above commonly used microscopic techniques because of no requirement for complex sample preparation by fixation or fluorescent-dye staining. Here, we employed the PT technique using a simple diode laser to visualize skeletal muscle mitochondria in unstained and stained tissues. The fine mitochondrial network structures in muscle fibers could be imaged with the PT imaging system, even in unstained tissues. PT imaging of tissues stained with toluidine blue revealed the structures of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria and the swelling behavior of mitochondria in damaged muscle fibers with sufficient image quality. PT image analyses based on fast Fourier transform (FFT) and Grey-level co-occurrence matrix (GLCM) were performed to derive the characteristic size of mitochondria and to discriminate the image patterns of normal and damaged fibers.

Original languageEnglish
Article number#284859
JournalBiomedical Optics Express
Issue number6
Publication statusPublished - 2017 Jun 1


  • Imaging systems
  • Medical optics and biotechnology
  • Photothermal effects

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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