Photorepair of RNA polymerase arrest and apoptosis after ultraviolet irradiation in normal and XPB deficient rodent cells

V. Chiganças, L. F Z Batista, G. Brumatti, G. P. Amarante-Mendes, A. Yasui, C. F M Menck

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Cyclobutane pyrimidine dimers (CPDs) are directly involved in signaling for UV-Induced apoptosis in mammalian cells. Failure to remove these lesions, specially those located at actively expressing genes, is critical, as cells defective in transcription coupled repair have increased apoptotic levels. Thus, the blockage of RNA synthesis by lesions is an important candidate event triggering off active cell death. In this work, wild-type and XPB mutated Chinese hamster ovary (CHO) cells expressing a marsupial photolyase, that removes specifically CPDs from the damaged DNA, were generated, in order to investigate the importance of this lesion in both RNA transcription blockage and apoptotic induction. Photorepair strongly recovers RNA synthesis in wild-type CHO cell line, although the resumption of transcription is decreased in XPB deficient cells. This recovery is accompanied by the prevention of cells entering into apoptosis. These results demonstrate that marsupial photolyase has access to CPDs blocking RNA synthesis in vivo, and this may be affected by the presence of a mutated XPB protein.

Original languageEnglish
Pages (from-to)1099-1107
Number of pages9
JournalCell Death and Differentiation
Volume9
Issue number10
DOIs
Publication statusPublished - 2002 Oct 1
Externally publishedYes

Keywords

  • Apoptosis
  • Cyclobutane pyrimidine dimers
  • Photoreactivation
  • RNA transcription

ASJC Scopus subject areas

  • Cell Biology

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