TY - JOUR
T1 - Photorepair of RNA polymerase arrest and apoptosis after ultraviolet irradiation in normal and XPB deficient rodent cells
AU - Chiganças, V.
AU - Batista, L. F Z
AU - Brumatti, G.
AU - Amarante-Mendes, G. P.
AU - Yasui, A.
AU - Menck, C. F M
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Cyclobutane pyrimidine dimers (CPDs) are directly involved in signaling for UV-Induced apoptosis in mammalian cells. Failure to remove these lesions, specially those located at actively expressing genes, is critical, as cells defective in transcription coupled repair have increased apoptotic levels. Thus, the blockage of RNA synthesis by lesions is an important candidate event triggering off active cell death. In this work, wild-type and XPB mutated Chinese hamster ovary (CHO) cells expressing a marsupial photolyase, that removes specifically CPDs from the damaged DNA, were generated, in order to investigate the importance of this lesion in both RNA transcription blockage and apoptotic induction. Photorepair strongly recovers RNA synthesis in wild-type CHO cell line, although the resumption of transcription is decreased in XPB deficient cells. This recovery is accompanied by the prevention of cells entering into apoptosis. These results demonstrate that marsupial photolyase has access to CPDs blocking RNA synthesis in vivo, and this may be affected by the presence of a mutated XPB protein.
AB - Cyclobutane pyrimidine dimers (CPDs) are directly involved in signaling for UV-Induced apoptosis in mammalian cells. Failure to remove these lesions, specially those located at actively expressing genes, is critical, as cells defective in transcription coupled repair have increased apoptotic levels. Thus, the blockage of RNA synthesis by lesions is an important candidate event triggering off active cell death. In this work, wild-type and XPB mutated Chinese hamster ovary (CHO) cells expressing a marsupial photolyase, that removes specifically CPDs from the damaged DNA, were generated, in order to investigate the importance of this lesion in both RNA transcription blockage and apoptotic induction. Photorepair strongly recovers RNA synthesis in wild-type CHO cell line, although the resumption of transcription is decreased in XPB deficient cells. This recovery is accompanied by the prevention of cells entering into apoptosis. These results demonstrate that marsupial photolyase has access to CPDs blocking RNA synthesis in vivo, and this may be affected by the presence of a mutated XPB protein.
KW - Apoptosis
KW - Cyclobutane pyrimidine dimers
KW - Photoreactivation
KW - RNA transcription
UR - http://www.scopus.com/inward/record.url?scp=0036790477&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036790477&partnerID=8YFLogxK
U2 - 10.1038/sj.cdd.4401072
DO - 10.1038/sj.cdd.4401072
M3 - Article
C2 - 12232798
AN - SCOPUS:0036790477
VL - 9
SP - 1099
EP - 1107
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
SN - 1350-9047
IS - 10
ER -