Photodynamic Therapy of Intestinal Tumors with Mono-L-aspartyl Chlorin e6 (NPe6): A Basic Study

Tatsuya Kikuchi, Tohru Asakura, Hiroyuki Aihara, Manabu Shiraki, Sho Takagi, Yoshitaka Kinouchi, Katsuo Aizawa, Toru Shimosegawa

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5 Citations (Scopus)

Abstract

Background: To clarify the usefulness of the second-generation photosensitizer, mono-L-aspartyl chlorin e6 (NPe6), we examined the possibility of photodynamic diagnosis and photodynamic therapy for intestinal tumors in a mouse model. Materials and Methods: NPe6 was intravenously administered to the tumor-bearing mice through the tail vein. The intestinal tumor sites were irradiated with a 664-nm diode laser at constant intervals after the administration of photosensitizers. The tumors were excised and fluorescence was observed in frozen sections by microscope. Results: We observed the fluorescent image and calculated that the mean fluorescence intensity was significantly higher in the tumors than the normal mucosa during 6 hours (p<0.05). The fluorescence of NPe6 was chiefly accumulated in the intestinal tumors as red fluorescence on the fluorescent microphotographic image. Conclusion: We conclude that NPe6 may be a valuable photosensitizer for the photodynamic diagnosis and photodynamic therapy of intestinal tumors.

Original languageEnglish
Pages (from-to)4897-4900
Number of pages4
JournalAnticancer research
Volume23
Issue number6 C
Publication statusPublished - 2003 Nov 1

Keywords

  • Mono-L-aspartyl chlorin e6
  • Photodynamic diagnosis
  • Photodynamic therapy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Kikuchi, T., Asakura, T., Aihara, H., Shiraki, M., Takagi, S., Kinouchi, Y., Aizawa, K., & Shimosegawa, T. (2003). Photodynamic Therapy of Intestinal Tumors with Mono-L-aspartyl Chlorin e6 (NPe6): A Basic Study. Anticancer research, 23(6 C), 4897-4900.