TY - JOUR
T1 - Photo-immobilization of a phospholipid polymer for surface modification
AU - Konno, Tomohiro
AU - Hasuda, Hirokazu
AU - Ishihara, Kazuhiko
AU - Ito, Yoshihiro
N1 - Funding Information:
This work was supported in part by a grant from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (14380406). The authors thank Mr. Akihiko Watanabe for his support with the AFM measurement.
PY - 2005/4
Y1 - 2005/4
N2 - A photo-reactive polymer having a phospholipid polar group was prepared, and the polymer was photo-immobilized on polymeric surfaces, where its interactions with biocomponents were investigated. By using a photo-immobilization method, the polymer was used for surface modification of polyethylene and polypropylene, polymers whose surfaces were not treated in our previous development of the phosphorylcholine-derived polymer. The photo-reactive polymer was synthesized by a coupling reaction involving copolymer consisting of 2-methacryloyloxyethyl phosphorylcholine and methacrylic acid with 4-azidoaniline. When the polymer was unpattern immobilized on the surface, X-ray photo-electron spectroscopic analysis and static contact angle measurements were performed. It was shown that the surface was covered with phospholipid polar groups. Micropattern immobilization was carried out using a micropatterned photo-mask. Measurements using atomic force microscopy showed that the swelled micropatterned polymer was five times as thick as the dried one. Protein adsorption and platelet adhesion were reduced on the polymer-immobilized regions. Mammalian cells did not adhere, and formed aggregates on the immobilized regions. In conclusion, the photo-reactive phospholipid polymer was covalently immobilized on the conventional polymer surfaces and it tended to reduce interactions with proteins and cells.
AB - A photo-reactive polymer having a phospholipid polar group was prepared, and the polymer was photo-immobilized on polymeric surfaces, where its interactions with biocomponents were investigated. By using a photo-immobilization method, the polymer was used for surface modification of polyethylene and polypropylene, polymers whose surfaces were not treated in our previous development of the phosphorylcholine-derived polymer. The photo-reactive polymer was synthesized by a coupling reaction involving copolymer consisting of 2-methacryloyloxyethyl phosphorylcholine and methacrylic acid with 4-azidoaniline. When the polymer was unpattern immobilized on the surface, X-ray photo-electron spectroscopic analysis and static contact angle measurements were performed. It was shown that the surface was covered with phospholipid polar groups. Micropattern immobilization was carried out using a micropatterned photo-mask. Measurements using atomic force microscopy showed that the swelled micropatterned polymer was five times as thick as the dried one. Protein adsorption and platelet adhesion were reduced on the polymer-immobilized regions. Mammalian cells did not adhere, and formed aggregates on the immobilized regions. In conclusion, the photo-reactive phospholipid polymer was covalently immobilized on the conventional polymer surfaces and it tended to reduce interactions with proteins and cells.
KW - Cell adhesion
KW - Phospholipid polymer
KW - Photo-immobilization
KW - Protein adsorption
KW - Surface modification
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U2 - 10.1016/j.biomaterials.2004.04.047
DO - 10.1016/j.biomaterials.2004.04.047
M3 - Article
C2 - 15482825
AN - SCOPUS:5044225696
VL - 26
SP - 1381
EP - 1388
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 12
ER -