Photo-assisted micro-gluing for assembling three-dimensional microstructures with living cells

Shotaro Yoshida, Koji Sato, Kaori Kuribayashi-Shigetomi, Tetsuhiko Teshima, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper describes a method to assemble three-dimensional (3D) microstructures by gluing separated two-dimensional (2D) structures attaching with living cells at micron-scales using hydrogels. We utilized photo-curable, biocompatible poly(ethylene)-glycol-diacrylate (PEGDA) as a glue, and assembled 2D cell-laden microplates into 3D structures by photoassisted local polymerizations. We demonstrated an assembly of 3D half-cubic microstructures with genetically-labeled cells, which enabled observing intracellular microstructure in 3D cellular constructs. We believe our method is useful for assembling 3D structures with targeted cells and into arbitrary geometries.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages509-511
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Keywords

  • 3D microstructure
  • Assembly
  • Cell handling
  • Gene expression
  • Hydrogel

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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