TY - JOUR
T1 - Photic induction of mPer1 and mPer2 in cry-deficient mice lacking a biological clock
AU - Okamura, Hitoshi
AU - Miyake, Shigeru
AU - Sumi, Yasuo
AU - Yamaguchi, Shun
AU - Yasui, Akira
AU - Muijtjens, Manja
AU - Hoeijmakers, Jan H.J.
AU - Van Der Horst, Gijsbertus T.J.
PY - 1999/12/24
Y1 - 1999/12/24
N2 - Mice lacking mCry1 and mCry2 are behaviorally arrhythmic. As shown here, cyclic expression of the clock genes mPer1 and mPer2 (mammalian Period genes 1 and 2) in the suprachiasmatic nucleus and peripheral tissues is abolished and mPer1 and mPer2 mRNA levels are constitutively high. These findings indicate that the biological clock is eliminated in the absence of both mCRY1 and mCRY2 (mammalian cryptochromes 1 and 2) and support the idea that mammalian CRY proteins act in the negative limb of the circadian feedback loop. The mCry double-mutant mice retain the ability to have mPer1 and mPer2 expression induced by a brief light stimulus known to phase-shift the biological clock in wild-type animals. Thus, mCRY1 and mCRY2 are dispensable for light-induced phase shifting of the biological clock.
AB - Mice lacking mCry1 and mCry2 are behaviorally arrhythmic. As shown here, cyclic expression of the clock genes mPer1 and mPer2 (mammalian Period genes 1 and 2) in the suprachiasmatic nucleus and peripheral tissues is abolished and mPer1 and mPer2 mRNA levels are constitutively high. These findings indicate that the biological clock is eliminated in the absence of both mCRY1 and mCRY2 (mammalian cryptochromes 1 and 2) and support the idea that mammalian CRY proteins act in the negative limb of the circadian feedback loop. The mCry double-mutant mice retain the ability to have mPer1 and mPer2 expression induced by a brief light stimulus known to phase-shift the biological clock in wild-type animals. Thus, mCRY1 and mCRY2 are dispensable for light-induced phase shifting of the biological clock.
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U2 - 10.1126/science.286.5449.2531
DO - 10.1126/science.286.5449.2531
M3 - Article
C2 - 10617474
AN - SCOPUS:0033601239
VL - 286
SP - 2531
EP - 2534
JO - Science
JF - Science
SN - 0036-8075
IS - 5449
ER -